The Macrophage Colony Stimulating Factor-1 Response Signature in Breast Carcinoma | Andrew H Beck, Inigo Espinosa, Badreddin Edris, Rui Li, Kelli Montgomery, Shirley Zhu, Sushama Varma, Robert J Marinelli, Matt van de Rijn, Robert B West <body bgcolor=white link="#000080" alink=red vlink="#000080"> <table width=800 cellspacing=6> <tr> <td width=200 valign=top> <center> <img src="topleft.gif"><p> <table width=150 cellpadding=4> <tr> <td bgcolor="#E0E0E0"> <font face="verdana, tahoma, arial"><font color="#000080"><center><a href="index.html" style="text-decoration: none; ">Home</a></font></font> </td> </tr> </table> <p> <table width=150 cellpadding=4> <tr> <td bgcolor="#E0E0E0"> <font face="verdana, tahoma, arial"><font color="#000080"><center><a href="figures.html" style="text-decoration: none;">Figures</a></font></font> </td> </tr> </table> <p> <table width=150 cellpadding=4> <tr> <td bgcolor="#E0E0E0"> <font face="verdana, tahoma, arial"><font color="#000080"><center><a href="images.html" style="text-decoration: none;">Histological Images</a></font> </font> </td> </tr> </table> <p> <table width=150 cellpadding=4> <tr> <td bgcolor="#E0E0E0"> <font face="verdana, tahoma, arial"><font color="#000080"><center><a href="data.html" style="text-decoration: none;">Supplementary Data</a></font></font> </td> </tr> </table> <p> <table width=150 cellpadding=4> <tr> <td bgcolor="#E0E0E0"> <font face="verdana, tahoma, arial"><font color="#000080"><center><A HREF="/tma_portal/" style="text-decoration: none;">WebPortal</A></font> </font> </td> </tr> </table> <p> <table width=150 cellpadding=4> <tr> <td bgcolor="#E0E0E0"> <font face="verdana, tahoma, arial"><font color="#000080"><center><a href="authors.html" style="text-decoration: none;">Authors</a></font></font> </td> </tr> </table> </td> <td valign=top> <center> <font face="verdana, tahoma, arial"> <font size=+2>The Macrophage Colony Stimulating Factor-1 Response Signature in Breast Carcinoma</font><p> Andrew H Beck*<sup>1</sup>, Inigo Espinosa*<sup>1</sup>, Badreddin Edris<sup>1</sup>, Rui Li<sup>1</sup>, Kelli Montgomery<sup>1</sup>, Shirley Zhu<sup>1</sup>, Sushama Varma<sup>1</sup>, Robert J Marinelli<sup>1</sup>, Matt van de Rijn<sup>1</sup>, Robert B West<sup>1,2</sup><p> <table width=100% cellpadding=4> <tr> <td bgcolor="#E0E0E0"> <font face="verdana, tahoma, arial"><font size=+1 color="#000080">Figures</font></font> </td> </tr> <tr> <td> <font face="verdana, tahoma, arial"> <font size=-1> <p> <b><a href="CSF1_CCR_FIG1-1.gif" style="text-decoration: none;">Figure 1.</a></b> Unsupervised Hierarchical Clustering of Breast Carcinomas with CSF1 Response Genes in Five Datasets. A, Perreard (n=91). B, GSE1379 (n=60). C, GSE1456 (n=159). D, GSE3494 (n=251), E, NKI (n=295). Within the heatmap, yellow represents high expression, black represents median expression, and blue represents low expression. The red highlighted region of the dendrograms above the heatmaps indicates the cluster of breast cancer cases with the CSF1 response signature in each dataset.<p> <center><a href="CSF1_CCR_FIG1-1.gif" style="text-decoration: none;"><img src="CSF1_CCR_FIG1-1.gif" width=500 border=0></a></center><p><br> <b><a href="Figure2_CSF1_CCR-1.gif" style="text-decoration: none;">Figure 2.</a></b> Kaplan Meier Survival Curves for Breast Cancer Cases Stratified by CSF1 Response Signature. A, The survival curves are displayed individually for each of the five datasets. B, The survival curves display data pooled from the five datasets for disease free survival, disease specific survival, and overall survival.<p> <center><a href="Figure2_CSF1_CCR-1.gif" style="text-decoration: none;"><img src="Figure2_CSF1_CCR-1.gif" width=500 border=0></center><p><br> <b><a href="Fig3_CSF1-CCR.gif" style="text-decoration: none;">Figure 3.</a></b> Kaplan Meier Survival Curves for CSF1 response Signature Subset Analyses. Kaplan Meier survival curves are displayed for breast cancer cases stratified by CSF1 response signature for subsets determined by: A, estrogen receptor status; B, grade; and C, p53 mutation signature.<p> <center><a href="Fig3_CSF1-CCR.gif" style="text-decoration: none;"><img src="Fig3_CSF1-CCR.gif" width=500 border=0></center><p><br> <b><a href="Fig4_CSF1_CCR.gif" style="text-decoration: none;">Figure 4.</a></b> Coordinate Expression of CSF1 and CSF1 Response Proteins in Breast Cancer. A, Unsupervised hierarchical clustering of 272 breast carcinomas based on tissue microarray staining for CSF1 and five CSF1 response markers (CSF1R, CD163, FCGR3a, FCGR2a, CTSL1). The breast cancer cases are arranged along the x axis above the heatmap, and CSF1 and the CSF1 response markers are arranged along the y axis to the right of the heatmap. The CSF1-E row represents CSF1 epithelial expression and the CSF1-S represents CSF1 stromal expression. The colorbar underlying the dendrogram summarizes the expression levels for all 5 CSF1 response markers and shows red for cases expressing at least 4 CSF1 response markers and green for cases expressing less than 4 CSF1 response markers. B, Example of a breast cancer case with stromal expression of CSF1 (in situ hybridization) and five CSF1 response proteins: CSF1R (in situ hybridization), and CD163, FCGR3a, FCGR2a, CTSL1 (immunohistochemistry).<p> <center><a href="Fig4_CSF1_CCR.gif" style="text-decoration: none;"><img src="Fig4_CSF1_CCR.gif" width=500 border=0></center><p><br> </td> </tr> </table> </td> </tr> </table> </body> </html>